讀懂GenBank文件格式中的資料
一、 LOCUS
在 GenBank 格式中，
LOCUS NM_001469 2156 bp mRNA linear （家系血統(tǒng)） PRI （ primate 猿類） 16-DEC-2004
DEFINITION Homo sapiens thyroid autoantigen 70kDa (Ku antigen) (G22P1), mRNA.
The LOCUS field contains a number of different data elements, including locus name, sequence length, molecule type, GenBank division, and modification date. Each element is described below.
二、 COMMENT
1 、 REVIEWED REFSEQ ：說明了該 RefSeq 生成的過程。
2 、 Summary ：說明了該序列的功能。
三、 Feature
名詞解釋： information about genes and gene products, as well as regions of （ biological significance reported in the ） sequence. These can include regions of the sequence that code for proteins and RNA molecules.
Feature 下的副標(biāo)題內(nèi)容太復(fù)雜，必要時(shí)到這里 The DDBJ/EMBL/GenBank Feature Table 查 .
1 、 key ：一般選擇 Location/Qualifier 。
2 、 complement ： cDNA 。 If a feature is located on the complementary strand, the word "complement" will appear before the base span.
3 、 5< ：指向 5 ’端。 If the "<" symbol precedes a base span, the sequence is partial on the 5' end (e.g., CDS <1..206). If the ">" symbol follows a base span, the sequence is partial on the 3' end (e.g., CDS 435..915>.
4 、 /db_xref ：其字符串是通往其他數(shù)據(jù)庫的鏈接。
/db_xref="taxon:9606" taxonomy 物種分類學(xué)
/db_xref="GeneID:2547" 鏈接到 Gene 。
/db_xref="LocusID:2547" 鏈接到 Locuslink 。
/db_xref="MIM:152690" 鏈接到 OMIM 。
四、兩個(gè)例子：
Key =Location/Qualifiers
CDS=23..400
====/product="alcohol dehydrogenase"
====/gene="adhI"
might be read as:
The feature CDS is a coding sequence beginning at base 23 and ending at base 400, has a product called 'alcohol dehydrogenase' and is coded for by a gene called “ adhI ”
A more complex description:
Key=Location/Qualifiers
CDS=join(544..589,688..>1032)
====/product="T-cell receptor beta-chain"
which might be read as:
This feature, which is a partial coding sequence is formed by joining elements indicated to form one contiguous sequence encoding a product called T-cell receptor beta-chain.
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